Conjunctivitis Research Today is a free monthly online journal that collates and summarizes the latest research about Conjunctivitis, including details on pink eye, symptoms, causes, treatment, medication. | ||||||||
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Association of toll-like receptor four single nucleotide polymorphisms with incidence of infectious bovine keratoconjunctivitis (IBK) in cattle.Kataria RS, Tait RG, Kumar D, Ortega MA, Rodiguez J, Reecy JM DNA Fingerprinting Unit, National Bureau of Animal Genetic Resources, Karnal, India. Toll-like receptor 4 (TLR4) is a receptor protein that binds pathogen ligands, which are mainly associated with Gram-negative bacteria. The objective of this study was to investigate the association of nucleotide polymorphisms in TLR4 with infectious bovine keratoconjunctivitis (IBK), or pinkeye, incidence in American Angus cattle. Animals with previously calculated breeding values for IBK susceptibility were used to identify two SNPs in TLR4; Int1 (A/G) in intron1 (-26 Ex2 position) and Ex3 (C/T) in exon3 (1,678 position). To investigate the possible role of these SNPs in IBK susceptibility, the disease incidence information was collected on 370 calves raised in Iowa at two time points-June or August (disease season) and October (at weaning) and genotyped using PCR-RFLP protocols. In statistical models including year, pasture management group, and SNP, the Int1 SNP had a significant effect on IBK infection rates both in-season (P < 0.05) and at weaning (P < 0.01), whereas the Ex3 SNP was not significant (P > 0.79) at either time point. Furthermore, the Int1 SNP alone could account for 2.1% of phenotypic variation in IBK infection during the disease season and 3.0% of phenotypic variation in IBK infection at the time of weaning. These data indicate that there is a relationship between Int1 genotype and the rate of IBK infection in American Angus cattle. Published 12 January 2011 in Immunogenetics, 63(2): 115-9.
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